- #INDUCED PLURIPOTENT STEM CELLS REVIEW FULL#
- #INDUCED PLURIPOTENT STEM CELLS REVIEW TRIAL#
- #INDUCED PLURIPOTENT STEM CELLS REVIEW FREE#
This review, encompassing the fundamental concepts of regenerative medicine, is intended to provide a comprehensive portrait of important progress in stem cell research and development. Specifically, we highlight the following crucial domains: 1) sources of pluripotent cells 2) next-generation in vivo direct reprogramming technology 3) cell types derived from PSCs and the influence of genetic memory 4) induction of pluripotency with genomic modifications 5) construction of vectors with reprogramming factor combinations 6) enhancing pluripotency with small molecules and genetic signaling pathways 7) induction of cell reprogramming by RNA signaling 8) induction and enhancement of pluripotency with chemicals 9) maintenance of pluripotency and genomic stability in induced pluripotent stem cells (iPSCs) 10) feeder-free and xenon-free culture environments 11) biomaterial applications in stem cell biology 12) three-dimensional (3D) cell technology 13) 3D bioprinting 14) downstream stem cell applications and 15) current ethical issues in stem cell and regenerative medicine. We begin by discussing experimental advances in the generation and differentiation of pluripotent stem cells (PSCs), next moving to the maintenance of stem cells in different culture types, and finishing with a discussion of three-dimensional (3D) cell technology and future stem cell applications. In this article, we provide a systemic overview of the major recent discoveries in this exciting and rapidly developing field. Science 324, 797-801.Over the past 20 years, and particularly in the last decade, significant developmental milestones have driven basic, translational, and clinical advances in the field of stem cell and regenerative medicine.
#INDUCED PLURIPOTENT STEM CELLS REVIEW FREE#
Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II & Thomson JA (2009) Human induced pluripotent stem cells free of vector and transgene sequences. (2014) Platform for induction and maintenance of transgene-free hiPSCs resembling ground state pluripotent stem cells. Valamehr B, Robinson M, Abujarour R, Rezner B, Vranceanu F, Le T, Medcalf A, Lee TT, Fitch M, Robbins D et al. (2019) Induced pluripotent stem cells and their use in human models of disease and development. Karagiannis P, Takahashi K, Saito M, Yoshida Y, Okita K, Watanabe A, Inoue H, Yamashita JK, Todani M, Nakagawa M et al. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K & Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K & Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. © 2021 Federation of European Biochemical Societies.
#INDUCED PLURIPOTENT STEM CELLS REVIEW TRIAL#
The next decades will see the further spread of iPS cell technology-based regenerative medicine.Ĭell therapy clinical trial disease modeling iPS cell stock project regenerative medicine.
#INDUCED PLURIPOTENT STEM CELLS REVIEW FULL#
Clinical medicine using iPS cells has advanced safely and effectively by making full use of current scientific standards, but tests on cell safety need to be further developed and validated. Next, we introduce disease models generated from patient iPS cells and successfully used to identify therapeutic agents for intractable diseases. Then, we describe iPS cell-based therapies in or approaching clinical application, including those for ophthalmological, neurological, cardiac, hematological, cartilage, and metabolic diseases. First, we discuss progress in the establishment of iPS cells, including the HLA-homo iPS cell stock project in Japan and the advancement of low antigenic iPS cells using genome-editing technology. In this review, we summarize these technologies and their clinical application. Regenerative medicine using iPS cell technologies has progressed remarkably in recent years.
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